Shenlian extract attenuates myocardial ischaemia-reperfusion injury via inhibiting M1 macrophage polarization by silencing miR-155

作者：Min Seok Song, Xihe Cui, Jing Zhang, Yujie Li, Jingjing Li, Yuanlong Zang, Qi Li, Qing Yang, Ying Chen, Weiyan Cai, Xiaogang Weng, Yajie Wang, Xiaoxin Zhu · 发表于：Pharmaceutical Biology · 年份：2022 · DOI：10.1080/13880209.2022.2117828 · 被引用次数：29 · 研究领域：Cardiac Fibrosis and Remodeling、Traditional Chinese Medicine Analysis、Atherosclerosis and Cardiovascular Diseases

Context Shenlian extract (SL) is a combination of Salvia miltiorrhiza Bge. (Labiatae) and Andrographis paniculata (Burm. F.) Wall. Ex Nees (Acanthaceae) extracts, which promote blood circulation and clear endogenous heat toxins. Myocardial ischaemia-reperfusion injury (MI/RI) is aggravated myocardial tissue damage induced by reperfusion therapy after myocardial infarction.Objectives This study explores the effect of SL on MI/RI and the underlying mechanism.Materials and methods Primary peritoneal macrophages (pMACs) were treated with LPS and SL (5, 10 or 20 μg/mL) for 24 h. The myocardial ischaemia-reperfusion (MI/R) model was established after administration of different doses of SL (90, 180 or 360 mg/kg). Myocardial tissue injury was assessed by methylthiazolyl tetrazolium (TTC) staining and levels of creatine kinase (CK), lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in mice. The double immunofluorescence staining of iNOS/F4/80 and CD86/F4/80 was used to detect macrophage M1 polarization. The levels of miR-155, inflammatory factors and chemokines were detected by qRT-PCR or ELISA. CD86, iNOS, SOCS3, JAK2, p-JAK2, STAT3 and p-STAT3 proteins expressions in macrophages were analyzed by western blotting. Conditioned medium transfer systems were designed to unite M1 macrophages with H/R cardiomyocytes, and cell apoptosis was detected by TUNEL staining, western blotting or immunohistochemistry.Results SL reduced apoptosis, diminished CK and LDH levels, raised SOD co...