Metabolite profiling of human‐originated Lachnospiraceae at the strain level

作者：Rashidin Abdugheni, Wen-Zhao Wang, Yujing Wang, Meng‐Xuan Du, Feng‐Lan Liu, Nan Zhou, Cheng‐Ying Jiang, Chang‐Yu Wang, Linhuan Wu, Juncai Ma, Chang Liu, Shuang‐Jiang Liu · 发表于：iMeta · 年份：2022 · DOI：10.1002/imt2.58 · 被引用次数：192 · 研究领域：Gut microbiota and health、Metabolomics and Mass Spectrometry Studies、Probiotics and Fermented Foods

Abstract The human gastrointestinal (GI) tract harbors diverse microbes, and the family Lachnospiraceae is one of the most abundant and widely occurring bacterial groups in the human GI tract. Beneficial and adverse effects of the Lachnospiraceae on host health were reported, but the diversities at species/strain levels as well as their metabolites of Lachnospiraceae have been, so far, not well documented. In the present study, we report on the collection of 77 h uman‐originated L a ch nospiraceae sp ecies (please refer hLchsp, https://hgmb.nmdc.cn/subject/lachnospiraceae ) and the in vitro metabolite profiles of 110 Lachnospiraceae strains ( https://hgmb.nmdc.cn/subject/lachnospiraceae/metabolites ). The Lachnospiraceae strains in hLchsp produced 242 metabolites of 17 categories. The larger categories were alcohols (89), ketones (35), pyrazines (29), short (C2–C5), and long (C > 5) chain acids (31), phenols (14), aldehydes (14), and other 30 compounds. Among them, 22 metabolites were aromatic compounds. The well‐known beneficial gut microbial metabolite, butyric acid, was generally produced by many Lachnospiraceae strains, and Agathobacter rectalis strain Lach‐101 and Coprococcus comes strain NSJ‐173 were the top 2 butyric acid producers, as 331.5 and 310.9 mg/L of butyric acids were produced in vitro, respectively. Further analysis of the publicly available cohort‐based volatile‐metabolomic data sets of human feces revealed that over 30% of the prevailing volatile metabo...