Different adaptive patterns of wheat with different drought tolerance under drought stresses and rehydration revealed by integrated metabolomic and transcriptomic analysis

作者：Liangjie Lv, Xiyong Chen, Hui Li, Jinan Huang, Yuping Liu, Aiju Zhao · 发表于：Frontiers in Plant Science · 年份：2022 · DOI：10.3389/fpls.2022.1008624 · 被引用次数：41 · 研究领域：Plant Stress Responses and Tolerance、Plant responses to water stress、Rice Cultivation and Yield Improvement

Wheat as a staple food crop is enduring ever-frequent intermittent and changing drought with the climate change. It is of great significance to highlight the adaptive approaches under such variable conditions at multiple levels to provide a comprehensive understanding of drought tolerance and facilitate the genetic breeding of wheat. Therefore, three wheat lines with different drought tolerance (drought-tolerant mutant Mu > common wheat CK > drought susceptible mutant mu ) were analyzed under moderate and severe drought stresses as well as rehydration. Samples were subjected to transcriptomic and metabolomic profiling in combination with physiological and biochemical determination. The moderate drought stress rendered 198 and 115 differentially expressed metabolites (DEMs) in CK and Mu , respectively. The severe drought stress rendered 166, 151 and 137 DEMs in CK, Mu and mu , respectively. The rehydration rendered 150 and 127 DEMs in CK and Mu . 12,557 and 10,402 differentially expressed genes (DEGs) were identified for CK and Mu under moderate drought stress, respectively. 9,893, 7,924, and 9,387 DEGs were identified for CK, Mu , and mu under severe drought stress, respectively. 13,874 and 14,839 were identified in CK and Mu under rehydration, respectively. Metabolomics results showed that amino acid was the most differentially expressed metabolites, followed by phenolic acids. Flavonoids played an important role in drought tolerance. Most enriched pathways und...