Effects of dietary collagen cofactors and hydroxyproline on the growth performance, textural properties and collagen deposition in swim bladder of Nibea coibor based on orthogonal array analysis

作者：Haoji Guo, Xin Liu, Mengdu Tian, Guoquan Liu, Yuying Yuan, Xiaokang Ye, Haoran Zhang, Lanfei Xiao, Shuqi Wang, Yucong Hong, Kai-Hui Sun, Fan Lin, Xiaobo Wen · 发表于：Aquaculture Reports · 年份：2022 · DOI：10.1016/j.aqrep.2022.101375 · 被引用次数：11 · 研究领域：Collagen: Extraction and Characterization、Marine Biology and Environmental Chemistry、Aquaculture Nutrition and Growth

The global demand for collagen is continuously growing over years. Nutritional regulation of quality and collagen deposition in the swim bladder, an ideal collagen source of marine product, is a promising method for improving the economic value of fish. In this study, to enhance growth efficiency and collagen deposition in swim bladder of an important economic marine fish, the chu’s croaker (Nibea coibor), a L9(34) orthogonal array was designed with three levels for each factor, which are hydroxyproline (Hyp) and the key cofactors for proline hydroxylation during collagen post-translation modification, such as A1-A3 (Hyp): 0, 10 and 20 g/kg; B1-B3 (vitamin C, VC): 0, 0.20 and 0.40 g/kg; C1-C3 (Fe2+): 0, 0.20 and 0.40 g/kg; D1-D3 (α-ketoglutarate, AKG): 0, 10 and 20 g/kg, to formulate the trial feeds. An 8-week feed trial was carried out on 405 fish (initial weight 269.04 ± 3.18 g) which were randomly distributed into the nine treatment groups with three replicates of 15 fish per cage. Results indicated that appropriate increase of dietary Hyp and VC level is more beneficial to collagen deposition and textural properties in swim bladder, while Hyp and AKG exerted more significant effects on the growth performance. Gene expression analysis of collagen metabolism indicated that Hyp mainly improved the synthesis of type I collagen and inhibited collagen degradation, while VC and AKG mainly play an important role in hydroxylation of proline residues on collagen. The best combinati...