Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis

作者：Xiaola Guo, Shuai Wang, Junmei Zhang, Rui Li, Yong’e Zhang, Zhengrong Wang, Qingming Kong, William C. Cho, Xianghong Ju, Yujuan Shen, Lingqiang Zhang, Haining Fan, Jianping Cao, Yadong Zheng · 发表于：PLoS neglected tropical diseases · 年份：2022 · DOI：10.1371/journal.pntd.0010814 · 被引用次数：16 · 研究领域：Parasitic infections in humans and animals、Extracellular vesicles in disease、Toxoplasma gondii Research Studies

Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis.