A single-cell atlas of the cycling murine ovary

作者：Mary E. Morris, Marie-Charlotte Meinsohn, Maëva Chauvin, Hatice D. Saatcioglu, A Kashiwagi, Natalie Sicher, Ngoc Minh Nguyen, Selena Yuan, Rhian Stavely, Minsuk Hyun, Patricia K. Donahoe, Bernardo L. Sabatini, David Pépin · 发表于：eLife · 年份：2022 · DOI：10.7554/elife.77239 · 被引用次数：102 · 研究领域：Single-cell and spatial transcriptomics、Reproductive System and Pregnancy、Reproductive Biology and Fertility

The estrous cycle is regulated by rhythmic endocrine interactions of the nervous and reproductive systems, which coordinate the hormonal and ovulatory functions of the ovary. Folliculogenesis and follicle progression require the orchestrated response of a variety of cell types to allow the maturation of the follicle and its sequela, ovulation, corpus luteum formation, and ovulatory wound repair. Little is known about the cell state dynamics of the ovary during the estrous cycle and the paracrine factors that help coordinate this process. Herein, we used single-cell RNA sequencing to evaluate the transcriptome of >34,000 cells of the adult mouse ovary and describe the transcriptional changes that occur across the normal estrous cycle and other reproductive states to build a comprehensive dynamic atlas of murine ovarian cell types and states.