Dynamics of the gut microbiota in rats after hypobaric hypoxia exposure

作者：Yang Han, Jiayu Xu, Yan Yan, Xiaojing Zhao · 发表于：PeerJ · 年份：2022 · DOI：10.7717/peerj.14090 · 被引用次数：21 · 研究领域：Gut microbiota and health、Metabolomics and Mass Spectrometry Studies、High Altitude and Hypoxia

Background Gut microbiota plays an important role in host health and is influenced by multiple factors. Hypobaric hypoxia usually existing at high altitude conditions can adversely affect normal physiological functions. However, the dynamic changes of gut microbiota influenced by hypobaric hypoxia have not been elucidated. Methods In this study, we collected fecal samples from seven rats at 14 time points from entering the hypobaric chamber (eight time points) to leaving the chamber (six time points) and five rats served as normoxic controls. Metagenome sequencing was performed on all samples and the dynamics of taxa and functions were analyzed. Results We found that the α -diversity was changed in the first 5 days after entering or leaving the hypobaric chamber. The β -diversity analysis revealed that gut microbiota structure was significantly separated among 14 time points. After entering the chamber, the relative abundance of Bacteroides decreased and the most abundant genus turned into Prevotella . The abundance of Firmicutes and Bacteroidetes showed an opposite trend and both have a significant change within 5 days after entering or leaving the hypobaric hypoxia chamber. Some obligate anaerobic bacteria belonging to Desulfovibrio and Alistipes were significantly enriched after entering the chamber for 5 weeks, whereas Probiotics like Bifidobacterium and Lactococcus , and short-chain fatty acids producers like Butyrivibrio and Pseudobutyrivibrio were significantly enriche...