Genetic relationship and diversity of cultivars and breeding lines of tetraploid Italian ryegrass ( Lolium multiflorum  Lam.) and its hybrids with Lolium ‐ Festuca complex based on genome‐wide allele frequency

作者：Ken‐ichi Tamura, Takako Kiyoshi, Akito Kubota, Akira Arakawa, Masahiro Fujimori, Jun‐ichi Yonemaru · 发表于：Grassland Science · 年份：2022 · DOI：10.1111/grs.12387 · 被引用次数：4 · 研究领域：Turfgrass Adaptation and Management、Plant and fungal interactions、Plant Disease Resistance and Genetics

Abstract Genetic relatedness and diversity of 62 cultivars and breeding lines of tetraploid Italian ryegrass ( Lolium multiflorum Lam.; 39 accessions) and its interspecific hybrids, Festulolium (18 accessions), and hybrid ryegrass ( Lolium × hybridum Hausskn.; 5 accessions), mainly from Japan, were revealed based on 2,824 genome‐wide allele frequencies obtained by the genotyping by random amplicon sequencing‐direct (GRAS‐Di) method using bulk genomic DNA testing. Genomic composition of each accession was estimated according to the occurrence of 77,373 unique GRAS‐Di sequences in the reference population consisting of diploid Italian ryegrass, meadow fescue ( Festuca pratensis Huds.), and perennial ryegrass ( Lolium perenne L.). The high correlation coefficient (0.98) between the fescue‐specific reads ratio and the previously obtained f‐ratio of genomic in situ hybridization in Festulolium cultivars suggests the usefulness of this simple method. Both cluster analysis based on Nei's standard genetic distance ( D ST ) and principal component analysis (PCA) showed that groups were formed largely by species. However, the complex heritage of Lolium‐Festuca ( Festulolium ) materials could not be determined by species registration or breeding history alone. Some Festulolium accessions were closely related to Italian ryegrass, whereas some defined as Italian ryegrass may actually be interspecific hybrids. The high genetic diversity of Festulolium compared to Italian ryegrass and hybri...