PLOD2 high expression associates with immune infiltration and facilitates cancer progression in osteosarcoma

作者：Zhen Wang, Gentao Fan, Hao Zhu, Lingfeng Yu, Diankun She, Yanting Wei, Jianhao Huang, Tianhang Li, Shoubin Zhan, Shenkai Zhou, Yan Zhu, Yicun Wang, Xi Chen, Jianning Zhao, Guangxin Zhou · 发表于：Frontiers in Oncology · 年份：2022 · DOI：10.3389/fonc.2022.980390 · 被引用次数：22 · 研究领域：Ferroptosis and cancer prognosis、Immune cells in cancer、Connective tissue disorders research

Background Osteosarcoma (OS) is the most common primary malignant bone tumors in children and adolescents. Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2) is a key gene in mediating the formation of the stabilized collagen cross-link, playing an important role in the progression of cancer. However, the interaction between OS and PLOD2 has not been clarified so far. Methods The target gene PLOD2 was screened through our own RNA-seq results and other two RNA-seq results from GEO database. The expression of PLOD2 in OS was detected by RT-qPCR, Western blot and immunohistochemistry. Functional experiments were performed to investigate the role of PLOD2 in OS cell invasion, migration and angiogenesis in vitro . An OS lung metastasis model was established to investigate the function of PLOD2 in OS metastasis and angiogenesis in vivo . The role of PLOD2 in immune infiltration in OS was explored by KEGG/GO analysis and immune infiltration analysis with TARGET, TCGA and TIMER. Results PLOD2 was high-expressed in OS, which was related to poor prognosis of OS patients. PLOD2 promoted OS cell migration, invasion and angiogenesis in vitro and aggravated OS metastasis and angiogenesis in vivo . Bioinformatic analysis showed that PLOD2 played an important role in immune cell infiltration in OS, including CD8 positive T cells, macrophages M0 cells, DC cells, endothelial cells, iDC cells, ly endothelial cells, MEP cells, mv endothelial cells, native B cells, smooth muscle cells and...