Expression, oncological and immunological characterizations of BZW1/2 in pancreatic adenocarcinoma

作者：Jiachen Ge, Senmao Mu, Erwei Xiao, Guangjin Tian, Lianyuan Tao, Deyu Li · 发表于：Frontiers in Genetics · 年份：2022 · DOI：10.3389/fgene.2022.1002673 · 被引用次数：18 · 研究领域：RNA modifications and cancer、Ferroptosis and cancer prognosis、Pancreatic and Hepatic Oncology Research

Background: Despite the progress in early diagnosis and treatment, prognosis of pancreatic adenocarcinoma (PAAD) is still poor. Basic leucine zipper and W2 domain-containing protein 1 (BZW1) and protein 2 (BZW2) are attached to the basic leucine zipper (bZIP) superfamily. Recently, BZW1 was identified as an important role in glycolysis of PAAD. However, the comprehensive reports about BZW1/2 in PAAD are not sufficient. Methods: RNA-seq data in the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were retrospectively analyzed. We explored the expression of BZW1/2 in PAAD tissues and the associations between BZW1/2 and prognosis. In addition, the potential roles of BZW1/2 in tumor microenvironment (TME) of PAAD were analyzed. Finally, clinicopathological data of 49 patients with PAAD in our institution were collected. Immunohistochemistry was used to determine the expression of BZW1/2 in PAAD samples. Results: BZW1 and BZW2 were upregulated in PAAD tissues compared to normal tissues ( p < 0.05). The expression of BZW1/2 were not significantly correlated with gender, grade and stage of PAAD ( p > 0.05). High expression of BZW2 was an independent predictor for poor prognosis of PAAD (HR 1.834, 95%CI 1.303–2.581, p = 0.001). And a nomogram to predict overall survival (OS) of PAAD was established with a C-index of 0.685. BZW1 and BZW2 expression were positively associated with T cell mediated immune response to tumor cell and Th2 cells in xCell d...