Somatic mutations during rapid clonal domestication of Populus alba var. pyramidalis

作者：Zeyu Zheng, Hongyin Hu, Weixiao Lei, Jin Zhang, Mingjia Zhu, Ying Li, Xu Zhang, Jianchao Ma, Dongshi Wan, Tao Ma, Guangpeng Ren, Dafu Ru · 发表于：Evolutionary Applications · 年份：2022 · DOI：10.1111/eva.13486 · 被引用次数：13 · 研究领域：CRISPR and Genetic Engineering、Genetically Modified Organisms Research、Evolution and Genetic Dynamics

Abstract For many clonally propagated species, the accumulation of somatic mutations is the principal driver of declines in yield and quality. However, somatic mutations may also promote genetic diversification. Thus, elucidating somatic mutation rates and patterns is important to understand the genetic basis undergirding the emergence of commercially valuable traits and developmental processes. In this study, we studied the effect of short‐time clonal domestication of Populus alba var. pyramidalis , a species that has been propagated by cutting for the last 67 years. We found that: (1) the somatic mutation rate for P. alba var. pyramidalis is 9.24 × 10 −9 , which is higher than rates observed in related species; (2) there were more mutations near heterozygous regions, and a larger proportion of CpG and CHG sites were associated with somatic mutations, which may be related to the blocking of DNA repair by methylation; and (3) deleterious mutations were not shared by multiple individuals, and all occurred in heterozygous states, demonstrating the strong selective pressures that act against deleterious mutations. Taken together, the results of our study provide a global view of somatic mutation that will aid efforts to understand the genetic basis of commercially valuable traits and to improve clonally breeding species.