Invitro Efficacy Testing of Bacteriocin against Multidrug Resistant Pseudomonas aeruginosa

作者：Kavi Kumar, Bhuvaneshwari Gunasekar, P. Neelusree · 发表于：International Journal of Current Microbiology and Applied Sciences · 年份：2022 · DOI：10.20546/ijcmas.2022.1105.013 · 被引用次数：1 · 研究领域：Probiotics and Fermented Foods、Medicinal Plants and Neuroprotection、Identification and Quantification in Food

Bacteriocin is a protein substance possessing antimicrobial activities during the growth by great number of Gram positive and Gram negative bacteria (Zacharof and Lovitt). It will destabilize the cytoplasmic membrane and kills bacteria. Various studies reported antibacterial activity of bacteriocin against Listeria and other Gram positive bacteria. Hence, there is a need to know the effectiveness of bacteriocin against drug resistant Gram negative bacteria especially Pseudomonas aeruginosa, the leading cause of Hospital Acquired Infection (HAI). Objective of the research was to find the efficacy testing of bacteriocin against multidrug resistant Pseudomonas aeruginosa, to isolate and identify multidrug resistant Pseudomonas aeruginosa. To extract and purify bacteriocin from lactobacillus acidophilus and to perform invitro efficacy testing of bacteriocin against multidrug resistant Pseudomonas aeruginosa. Conventional identification and antimicrobial susceptibility was performed based on conventional procedure. The study was done at the clinical microbiology laboratory of Saveetha Medical College and hospital during the period of January to June 2020. Total 60 samples were collected from both male and female and from all the age groups. Among that 60 samples 39% were woundswab, 29% were urine, 5% were pus, 3% were tissue culture, 3% of samples were tracheal culture. The strains of Pseudomonas aeruginosa collected for this study shows resistance to cefepime, ceftazidime, ciprof...