Exosomes from Artesunate-Treated Bone Marrow-Derived Mesenchymal Stem Cells Transferring SNHG7 to Promote Osteogenesis Via TAF15-RUNX2 Pathway

作者：Ming-Zhi Huang, Hongyan Chen, Guoxuan Peng, Hong Sun, Hong-Cheng Peng, Hai-Yang Li, Xiang-Hui Liu, Qing Li · 发表于：Regenerative Medicine · 年份：2022 · DOI：10.2217/rme-2022-0065 · 被引用次数：23 · 研究领域：MicroRNA in disease regulation、Extracellular vesicles in disease、Circular RNAs in diseases

Aim: Effect of artesunate (ART)-treated bone marrow-derived mesenchymal stem cells-derived exosomes (BMSC-Exos) on osteogenesis and its underlying mechanisms were investigated. Materials & methods: Proliferation, alkaline phosphatase activity and calcified nodule formation of osteoblasts were determined. A mouse model of osteoporosis was established by ovariectomy. Results: SNHG7 was upregulated in BMSC-Exos by twofold, which was further enhanced in ART-BMSC-Exos by about twofold. ART intensified BMSC-Exos-induced proliferation, alkaline phosphatase activity by about fourfold, calcified nodule formation by about threefold and upregulation of osteogenesis related molecules RUNX2 (by 50%), BMP2 (by 30%) and ATF4 (by 40%) via delivering SNHG7. Mechanistically, SNHG7 recruited TAF15 to facilitate RUNX2 stability. Conclusion: ART-BMSC-Exos facilitated osteogenesis via delivering SNHG7 by modulating TAF15/RUNX2 axis.