Oral biofilm dysbiosis during experimental periodontitis

作者：Apoena Aguiar Ribeiro, Yizu Jiao, Mustafa Girnary, Tomaz Alves, Liang Chen, Anna Farrell, Di Wu, Flavia Teles, Naohiro Inohara, Karen V. Swanson, Julie T. Marchesan · 发表于：Molecular Oral Microbiology · 年份：2022 · DOI：10.1111/omi.12389 · 被引用次数：23 · 研究领域：Oral microbiology and periodontitis research、Orthodontics and Dentofacial Orthopedics、Gut microbiota and health

OBJECTIVES: We have previously characterized the main osteoimmunological events that occur during ligature periodontitis. This study aims to determine the polymicrobial community shifts that occur during disease development. METHODS: Periodontitis was induced in C57BL/6 mice using the ligature-induced periodontitis model. Healthy oral mucosa swabs and ligatures were collected every 3 days from 0 to 18 days post-ligature placement. Biofilm samples were evaluated by 16SrRNA gene sequencing (Illumina MiSeq) and QIIME. Time-course changes were determined by relative abundance, diversity, and rank analyses (PERMANOVA, Bonferroni-adjusted). RESULTS: Microbial differences between health and periodontal inflammation were observed at all phylogenic levels. An evident microbial community shift occurred in 25 genera during the advancement of "gingivitis" (3-6 days) to periodontitis (9-18 days). From day 0 to 18, dramatic changes were identified in Streptococcus levels, with an overall decrease (54.04%-0.02%) as well an overall increase of Enterococcus and Lactobacillus (23.7%-73.1% and 10.1%-70.2%, respectively). Alpha-diversity decreased to its lowest at 3 days, followed by an increase in diversity as disease advancement. Beta-diversity increased after ligature placement, indicating that bone loss develops in response to a greater microbial variability (p = 0.001). Levels of facultative and strict anaerobic bacteria augmented over the course of disease progression, with a total of eigh...