VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

作者：Yang H, Fufangyu Zhao, Li Y, Xu Ming, Lianqing Li, Chuansong Wu, Hirokazu Miyoshi, Liu Y · 发表于：DOAJ (DOAJ: Directory of Open Access Journals) · 年份：2013 · 被引用次数：4 · 研究领域：Nanoparticle-Based Drug Delivery、RNA Interference and Gene Delivery、Polymer Surface Interaction Studies

Hong Yang,1 Fenglong Zhao,1 Ying Li,1 Mingming Xu,1 Li Li,1 Chunhui Wu,1 Hirokazu Miyoshi,2 Yiyao Liu11Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China; 2Radioisotope Research Center, University of Tokushima, Kuramoto-cho, Tokushima, JapanAbstract: Multifunctional nanomaterials with unique magnetic and luminescent properties have broad potential in biological applications. Because of the overexpression of vascular cell adhesion molecule-1 (VCAM-1) receptors in inflammatory endothelial cells as compared with normal endothelial cells, an anti-VCAM-1 monoclonal antibody can be used as a targeting ligand. Herein we describe the development of multifunctional core-shell Fe3O4@SiO2 nanoparticles with the ability to target inflammatory endothelial cells via VCAM-1, magnetism, and fluorescence imaging, with efficient magnetic resonance imaging contrast characteristics. Superparamagnetic iron oxide and fluorescein isothiocyanate (FITC) were loaded successfully inside the nanoparticle core and the silica shell, respectively, creating VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles that were characterized by scanning electron microscopy, transmission electron microscopy, fluorescence spectrometry, zeta potential assay, and fluorescence microscopy. The VCAM-1-targeted Fe3O4@SiO2(FITC) nanoparticles typically had a diameter of 355 ± 37 nm, showed superparamagnet...