Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin

作者：Tsutomu Kouyama, Koshin Mihashi · 发表于：European Journal of Biochemistry · 年份：1981 · DOI：10.1111/j.1432-1033.1981.tb06167.x · 被引用次数：720 · 研究领域：Lanthanide and Transition Metal Complexes、Polymer Surface Interaction Studies、Advanced MRI Techniques and Applications

A fluorescent reagent, N‐(1‐pyrenyl)iodoacetamide, was conjugated to rabbit skeletal muscle actin at the site of the most reactive sulfhydryl group, and fluorescence characteristics (excitation and emission spectra, quantum yields, lifetimes) of the conjugate were investigated. Associated with polymerization of labelled G‐actin, the fluorescence intensity at 407 nm, after excitation at 365 nm, was enhanced by a factor of about 25. It was reduced to about 25% on the binding of heavy meromyosin (or subfragment 1). The results suggest that binding of heavy meromyosin to the protomer of F‐actin alters the local structure of the protomer towards a G‐actin‐like one.