Influences of ferulic acid on intestinal digestive and antioxidant enzymes, immune, antioxidant gene and tight junction protein expression and microbiota in hybrid grouper (Epinephelus fuscoguttatus♀× Epinephelus polyphekadion♂)

作者：Wei-jie Fu, Eric Amenyogbe, Jun Luo, Er-jun Yang, Jiansheng Huang, You-Ming Chen, Gang Chen · 发表于：Aquaculture Reports · 年份：2022 · DOI：10.1016/j.aqrep.2022.101348 · 被引用次数：15 · 研究领域：Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth、Animal Nutrition and Physiology

The current study was performed to assess the effects of dietary administration of ferulic acid (FA) on digestive enzyme activities, antioxidant capacity, morphology, immunity, and microbiota in the gut of hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus polyphekadion♂). A total of 630 fish (9.51 ± 0.01 g) were allocated randomly to seven dietary treatments, each with three replicates. Experiment diets supplemented with FA at the levels of 0, 40, 80, 160, 320, 640, and 1280 mg kg−1 (FA0 (control), FA40, FA80, FA160, FA320, FA640, and FA1280), were fed to fish for 70 days. The results showed that dietary 80–160 mg kg−1 FA intake considerably augmented the enzymes activities of digestion (lipase, trypsin, amylase, and pepsin) and antioxidation (superoxide dismutase, glutathione peroxidase, and catalase) and the values of immune parameters (Immunoglobulin, C3 and lysozyme), as well as reduced malondialdehyde content, with respect to the control group (P < 0.05). Furthermore, a significant increase in the intestinal fold height, fold width, and muscular thickness was detected in fish fed the diets containing 80–160 mg kg−1 FA (P < 0.05). For gut genes expression, dietary supplementation of 80–160 mg kg−1 FA significantly upregulated the transcriptional levels of the immune-related genes (interleukin 1β, interleukin 8, tumor necrosis factor-alpha, interleukin 10, and transforming growth factor-beta 1), the antioxidation-associated genes (superoxide dismutase, copper/zinc s...