In vivo maternal haploid induction based on genome editing of DMP in Brassica oleracea

作者：Xinyu Zhao, Kaiwen Yuan, Yuxiang Liu, Nan Zhang, Limei Yang, Yangyong Zhang, Yong Wang, Jialei Ji, Zhiyuan Fang, Fengqing Han, Honghao Lv · 发表于：Plant Biotechnology Journal · 年份：2022 · DOI：10.1111/pbi.13934 · 被引用次数：43 · 研究领域：CRISPR and Genetic Engineering、Chromosomal and Genetic Variations、Plant tissue culture and regeneration

Brassica oleracea is an important plant species that includes many globally cultivated vegetable crops (cole crops), such as cabbage, broccoli, cauliflower, kale and Brussels sprouts. These plants provide human beings with not only plentiful nutrients such as carotenoids, minerals, vitamins A and C, dietary fibre but also unique health-promoting compounds like glucosinolates (Xu et al., 2014). Heterosis utilization in crops, including cole vegetables, requires the development of homozygous lines usually generated by multiple rounds of selfing or backcrossing (Zhong et al., 2019). Doubled haploid (DH) technology enables the generation of complete homozygous lines within two generations, dramatically accelerating the breeding progress (Zhong et al., 2020). However, traditional haploid induction (HI) in Brassica oleracea depends on an in vitro anther/microspore cultivation approach, which is not only complicated but also highly limited by plant genotype. In recent years, MTL/NLD/ZmPLA1, ZmDMP and ZmPOD65 were found to be responsible for inducing in vivo maternal haploid embryos in maize (Jiang et al., 2022 and references therein). Although orthologues of MTL/NLD/ZmPLA1 have not been found in dicots, ZmDMP-like genes are present in dicots and have been demonstrated to trigger in vivo maternal HI in Arabidopsis, Medicago truncatula, tomato, rapeseed and tobacco (Li et al., 2022; Wang et al., 2022; Zhong et al., 2020, 2022a,b). However, it is still not known whether this approach c...