Species classification and novel plasmid identifications in Arcobacter cryaerophilus and Arcobacter cryaerophilus-like organisms

作者：Guilan Zhou, Min Wang, Hairui Wang, Xiaoli Chen, Yixin Gu, Zhujun Shao, Jianzhong Zhang, Maojun Zhang · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.984450 · 被引用次数：6 · 研究领域：Metal Extraction and Bioleaching、Salmonella and Campylobacter epidemiology、Vibrio bacteria research studies

The Arcobacter is a globally emerging foodborne and zoonotic pathogen that can cause diarrhea in humans. It is relatively homogenous and clearly distinguishes the group from other Epsilonproteobacteria . Arcobacter cryaerophilus ( A. cryaerophilus ) is a heterogeneous species and little is known about its genomic characterization in China. This study aims to determine the genetic and plasmid features of A. cryaerophilus based on whole-genome sequence (WGS). Average Nucleotide Identity (ANI) and in silico DNA–DNA hybridization ( is DDH) were used for the species classification for 90 initially identified A. cryaerophilus strains. One complete genome and 42 draft genomes were obtained by whole genome sequencing. The genomic characteristics were determined using various bioinformatics software. The genomes of the strains examined were estimated to vary from 1.81 to 2.28 Mb in length, with a G + C content of around 27%. ANI and is DDH results indicated that 90 initially identified A. cryaerophilus strains should be reclassified into four new species (ANI > 96% or is DDH > 70%). Two clades (four subclades) were identified among 90 genomes with the phylogenetic analysis. The phylogenetic tree indicated these 90 genomes exhibited a high intra-species genomic diversity. No clustering was assorted with the host or geographic location among these genomes. Aminoglycoside resistance genes, such as aph(2’’)-Ih , AAC(6’)-Ie-APH(2’’)-Ia , aac(6’)-IIa , ant(6) , and streptothri...