Changes in amino acid and fatty acid composition during early development in cobia (Rachycentron canadum)

作者：Jiansheng Huang, Rongxin Li, Ruitao Xie, Youming Chen, Jiandong Zhang, Eric Amenyogbe, Gang Chen · 发表于：Frontiers in Marine Science · 年份：2022 · DOI：10.3389/fmars.2022.995616 · 被引用次数：11 · 研究领域：Aquaculture Nutrition and Growth、Reproductive biology and impacts on aquatic species、Aquaculture disease management and microbiota

An estimation of the utilization of endogenous nutrients from the yolk sac that occurs during embryonic and early larval development can be a valuable approach to studying the nutritional requirements of fish larvae. In this study, fertilized eggs, embryos (before the membrane, 24 hours after fertilization), newly-hatched larvae (0-d-old), and open-mouthed larvae (3-day-old, unfed rotifers) samples of cobia ( Rachycentron canadum ) were collected to determine the amino acid and fatty acid profiles. Crude protein composition varies significantly for cobia during the different stages of development. The total amino acids, except for lysine(Lys), histidine (His), methionine(Met), serine(Ser), and glycine(Gly), had no significant change in the early development stage ( P > 0.05); the content of other six essential amino acids (EAA) and six non-essential amino acids (NEAA) was consistent with the changing trend of total essential amino acid. From hatching newly hatched larvae to open-mouthed larvae, except for Leu, Phe and Ser, decreased significantly ( P <0.05). From the utilization rate of FAA, the utilization rate of Lys and Leu was the highest (60.26%) and (52.21%) in the embryonic and endogenous nutritional stages, respectively. The water content of the open-mouthed larvae of cobia was significantly higher than that of the fertilized eggs, embryos, and newly hatched larvae ( P < 0.05). Three kinds of saturated fatty acids (SFA), five kinds of monounsatura...