Transcriptome analysis of sweet potato responses to potassium deficiency

作者：Fang Wang, Wenfang Tan, Wei Song, Songtao Yang, Shuai Qiao · 发表于：BMC Genomics · 年份：2022 · DOI：10.1186/s12864-022-08870-5 · 被引用次数：33 · 研究领域：Plant nutrient uptake and metabolism、Plant Stress Responses and Tolerance、Plant Molecular Biology Research

Abstract Background As one of three essential nutrients, potassium is regarded as a main limiting factor for growth and development in plant. Sweet potato ( Ipomoea batatas L.) is one of seven major food crops grown worldwide, and is both a nutrient-rich food and a bioenergy crop. It is a typical ‘K-favoring’ crop, and the level of potassium ion (K + ) supplementation directly influences its production. However, little is known about the transcriptional changes in sweet potato genes under low-K + conditions. Here, we analyzed the transcriptomic profiles of sweet potato roots in response to K + deficiency to determine the effect of low-K + stress on this economically important crop. Results The roots of sweet potato seedlings with or without K + treatment were harvested and used for transcriptome analyses. The results showed 559 differently expressed genes (DEGs) in low and high K + groups. Among the DEGs, 336 were upregulated and 223 were downregulated. These DEGs were involved in transcriptional regulation, calcium binding, redox-signaling, biosynthesis, transport, and metabolic process. Further analysis revealed previously unknow genes involved in low-K + stress, which could be investigated further to improve low K + tolerance in plants. Confirmation of RNA-sequencing results using qRT-PCR displayed a high level of consistency between the two experiments. Analysis showed that many auxin-, ethylene- and jasmonic acid-related genes respond to K + deficiency, suggesting that t...