Human infection of avian influenza A H3N8 virus and the viral origins: a descriptive study

作者：Rengui Yang, Honglei Sun, Feng Gao, Kaiwei Luo, Zheng Y. X. Huang, Qi Tong, Hao Song, Qiqi Han, Jiyu Liu, Jiyu Liu, Yu Lan, Jianxun Qi, Han Li, Shuilian Chen, Mingzhong Xu, Jinsong Qiu, Zeng Ge, Xixing Zhang, Chaoyang Huang, Ruiqing Pei, Zhifei Zhan, Beiwei Ye, Yaxin Guo, Yinzhu Zhou, Wen Ye, Dong Yao, Min Ren, Bo Li, Jizhe Yang, Yanan Wang, Juan Pu, Yipeng Sun, Yi Shi, William J. Liu, Xinhua Ou, George F. Gao, Lidong Gao, Jinhua Liu, Jinhua Liu · 发表于：The Lancet Microbe · 年份：2022 · DOI：10.1016/s2666-5247(22)00192-6 · 被引用次数：177 · 研究领域：Influenza Virus Research Studies、Respiratory viral infections research、Infectious Encephalopathies and Encephalitis

BACKGROUND: The H3N8 avian influenza virus (AIV) has been circulating in wild birds, with occasional interspecies transmission to mammals. The first human infection of H3N8 subtype occurred in Henan Province, China, in April, 2022. We aimed to investigate clinical, epidemiological, and virological data related to a second case identified soon afterwards in Hunan Province, China. METHODS: We analysed clinical, epidemiological, and virological data for a 5-year-old boy diagnosed with H3N8 AIV infection in May, 2022, during influenza-like illness surveillance in Changsha City, Hunan Province, China. H3N8 virus strains from chicken flocks from January, 2021, to April, 2022, were retrospectively investigated in China. The genomes of the viruses were sequenced for phylogenetic analysis of all the eight gene segments. We evaluated the receptor-binding properties of the H3N8 viruses by using a solid-phase binding assay. We used sequence alignment and homology-modelling methods to study the effect of specific mutations on the human receptor-binding properties. We also conducted serological surveillance to detect the H3N8 infections among poultry workers in the two provinces with H3N8 cases. FINDINGS: The clinical symptoms of the patient were mild, including fever, sore throat, chills, and a runny nose. The patient's fever subsided on the same day of hospitalisation, and these symptoms disappeared 7 days later, presenting mild influenza symptoms, with no pneumonia. An H3N8 virus was is...