Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions

作者：Takakazu Kaneko, Shusei Sato, Haruru Kotani, Ayako Tanaka, E. Asamizu, Yasukazu Nakamura, Noriyuki Miyajima, M. Hirosawa, Masahiro Sugiura, Shigemi Sasamoto, Takaharu Kimura, Tsutomu Hosouchi, Ai Matsuno, A. Muraki, N. Nakazaki, K. Naruo, S. Okumura, Sayaka Shimpo, Chie Takeuchi, T. Wada, Akiko Watanabe, Manabu Yamada, Miho Yasuda, Sho Tabata · 发表于：DNA Research · 年份：1996 · DOI：10.1093/dnares/3.3.109 · 被引用次数：2159 · 研究领域：Algal biology and biofuel production、Microbial Community Ecology and Physiology、Protist diversity and phylogeny

The sequence determination of the entire genome of the Synechocystis sp. strain PCC6803 was completed. The total length of the genome finally confirmed was 3,573,470 bp, including the previously reported sequence of 1,003,450 bp from map position 64% to 92% of the genome. The entire sequence was assembled from the sequences of the physical map-based contigs of cosmid clones and of lambda clones and long PCR products which were used for gap-filling. The accuracy of the sequence was guaranteed by analysis of both strands of DNA through the entire genome. The authenticity of the assembled sequence was supported by restriction analysis of long PCR products, which were directly amplified from the genomic DNA using the assembled sequence data. To predict the potential protein-coding regions, analysis of open reading frames (ORFs), analysis by the GeneMark program and similarity search to databases were performed. As a result, a total of 3,168 potential protein genes were assigned on the genome, in which 145 (4.6%) were identical to reported genes and 1,257 (39.6%) and 340 (10.8%) showed similarity to reported and hypothetical genes, respectively. The remaining 1,426 (45.0%) had no apparent similarity to any genes in databases. Among the potential protein genes assigned, 128 were related to the genes participating in photosynthetic reactions. The sum of the sequences coding for potential protein genes occupies 87% of the genome length. By adding rRNA and tRNA genes, therefore, the g...