Monitoring mitochondrial nitroreductase activity in tumors and a hind-limb model of ischemia in mice using a novel activatable NIR fluorescent probe

作者：Xiaosheng Liu, Shuang Zeng, Ming Zhang, Maojun Jiang, Yves S. Kafuti, Pingping Shangguan, Yichu Yu, Qixian Chen, Jingyun Wang, Xiaojun Peng, Juyoung Yoon, Haidong Li · 发表于：Chemical Communications · 年份：2022 · DOI：10.1039/d2cc04112j · 被引用次数：34 · 研究领域：Cancer, Hypoxia, and Metabolism、Nanoplatforms for cancer theranostics、ATP Synthase and ATPases Research

We report a mitochondria-targeted nitroreductase (NTR)-activated near-infrared fluorescent probe: CS-NO2. Overexpressed NTR in mitochondria was measured with high sensitivity. More importantly, the probe CS-NO2 successfully monitored NTR activity in solid tumors and a hind-limb model of ischemia in mice. This novel finding indicates the promising function of our probe for the diagnosis of solid tumors and hypoxia-associated diseases.