CAMTA1 gene affects the ischemia-reperfusion injury by regulating CCND1

作者：Yang Liu, Guohui Shang, Xuran Zhang, Fuyong Liu, Chi Zhang, Zhihao Li, Jing Jia, Yan Xu, Zhaojing Zhang, Shangdong Yang, Baixue Zhou, Yingying Luan, Yanyang Huang, Peng Yue, Tianyi Han, Ying He, Hong Zheng · 发表于：Frontiers in Cellular Neuroscience · 年份：2022 · DOI：10.3389/fncel.2022.868291 · 被引用次数：13 · 研究领域：Epigenetics and DNA Methylation、Cancer-related molecular mechanisms research、Histone Deacetylase Inhibitors Research

Epigenetic modulations lead to changes in gene expression, including DNA methylation, histone modifications, and noncoding RNAs. In recent years, epigenetic modifications have been related to the pathogenesis of different types of cancer, cardiovascular disease, and other diseases. Emerging evidence indicates that DNA methylation could be associated with ischemic stroke (IS) and plays a role in pathological progression, but the underlying mechanism has not yet been fully understood. In this study, we used human methylation 850K BeadChip to analyze the differences in gene methylation status in the peripheral blood samples from two groups (3 IS patients vs. 3 healthy controls). According to their bioinformatics profiling, we found 278 genes with significantly different methylation levels. Seven genes with the most significant methylation modifications were validated in two expanded groups (100 IS patients vs. 100 healthy controls). The CAMTA1 gene had significantly different methylation changes in patients compared to the controls. To understand the CAMTA1 function in stroke, we generated CAMTA1 knockout in SH-SY5Y cells. RNA seq results in CAMTA1 knockout cells revealed the pathways and gene set enrichments involved in cellular proliferation and cell cycle. Furthermore, a series of experiments demonstrated that in the oxygen-glucose deprivation/re-oxygenation (OGD/R) model system, the expression of cyclin D1, an essential regulator of cell cycle progression, was increased in S...