Pathogenicity and antibiotic resistance analysis of Vibrio species found in coastal water at mainly beach of Shenzhen, China

作者：Ziwei Huang, Melody Abena Anokyewaa, Junlin Wang, Jichang Jian, Yishan Lu · 发表于：Frontiers in Marine Science · 年份：2022 · DOI：10.3389/fmars.2022.980593 · 被引用次数：7 · 研究领域：Vibrio bacteria research studies、Aquaculture disease management and microbiota、Identification and Quantification in Food

Offshore industries and resources are the links between humans and the marine ecosystem. Several risk factors are associated with beach activities. The risk of Vibrio infection at bathing beaches is often overlooked as there are virtually no regulations regarding them. In this study, we investigated the current prevalence of pathogenic Vibrio spp. on the main beaches of Shenzhen. The risk indicator levels of pathogenic Vibrio species obtained were analyzed using 17 virulence genes mainly associated with Vibrio species and are responsible for several ailments and infections. A total of 60 Vibrio strains were isolated and identified by morphological observation, evolutionary tree alignment, and biochemical testing. There was a high abundance of Vibrio in the seawater. Also, a positive correlation was observed between the presence of virulence genes and the exhibition of high pathogenicity after artificially infecting fish with some of the virulent Vibrio species. In the infection experiment, it was observed that all the zebrafish infected with MEDF7 ( Vibrio alginolyticus ) and JSW-YELLOW ( Vibrio harveyi ) died a day after injection, with varying degrees of abdominal enlargement and skin ulceration. The mortality rates for strains with medium risk and low risk were 65% and 45%, respectively. Lethal dose 50 (LD 50 ) values were 5.67 ×10 4 CFU/fish, 3.72 ×10 5 CFU/fish, and 4.31 ×10 5 CFU/fish for the high-risk, medium-risk, and low-risk strains of zebrafish, respectively. The r...