A composite score based on immune-related gene prognostic index and m6A risk score of head and neck squamous cell carcinoma

作者：Yizhou Yang, Zhixiong Lin, Zeman Cai, Kaichun Huang, Mei Li, Xiao Wang, Yinbing Lin, Sijie Chen, Zhining Yang · 发表于：Research Square · 年份：2022 · DOI：10.21203/rs.3.rs-1987322/v1 · 被引用次数：2 · 研究领域：RNA modifications and cancer、Cancer-related gene regulation、Epigenetics and DNA Methylation

Abstract Background: Immunotherapy has been demonstrated favorable in head and neck squamous cell carcinoma (HNSCC). Studies indicated that immune-related gene prognostic index (IRGPI) was a robust signature, and N6-methyladenosine (m 6 A) methylation had a significant impact on the tumor immune microenvironment (TIME) and immunotherapy of HNSCC. Thus, combining IRGPI with m 6 A status should offer a better predictive power for immune responses. Methods: HNSCC samples from The Cancer Genome Atlas (TCGA, n=498) and Gene Expression Omnibus database (GSE65858, n = 270) were used in this study. Cox regression analysis was used to construct the IRGPI through immune-related hub genes which were identified by weighted gene co-expression network analysis (WGCNA). The m 6 A risk score was constructed by least absolute shrinkage and selection operator regression analysis. Principal component analysis was used to construct a composite score, and systematically correlate subgroups according to TIME cell-infiltrating characteristics. Results: A composite score was determined based on IRGPI and m 6 A risk score. HNSCC patients in the TCGA were divided into four subgroups：A (IRGPI-High&m 6 A-risk-High, n=127), B (IRGPI-High&m 6 A-risk-Low, n=99), C (IRGPI-Low&m 6 A-risk-High, n=99) and D (IRGPI-Low&m 6 A-risk-Low, n=128), and overall survival (OS) was significantly different between subgroups ( P < 0.001). The characteristics of TIME cell infiltration in the four subgroup...