Comparative RNA-Seq analysis reveals insights in Salmonella disease resistance of chicken; and database development as resource for gene expression in poultry

作者：Mashooq Ahmad Dar, Syed Mudasir Ahmad, Basharat Bhat, Tanveer Ali Dar, Zulfqar ul Haq, Basharat A. Wani, Nadeem Shabir, Zahid Amin Kashoo, Riaz Ahmad Shah, Nazir Ahmad Ganai, Mohammad Heidari · 发表于：Genomics · 年份：2022 · DOI：10.1016/j.ygeno.2022.110475 · 被引用次数：28 · 研究领域：Salmonella and Campylobacter epidemiology、Animal Nutrition and Physiology、Aquaculture disease management and microbiota

Salmonella, one of the major infectious diseases in poultry, causes considerable economic losses in terms of mortality and morbidity, especially in countries that lack effective vaccination programs. Besides being resistant to diseases, indigenous chicken breeds are also a potential source of animal protein in developing countries. For understanding the disease resistance, an indigenous chicken line Kashmir faverolla, and commercial broiler were selected. RNA-seq was performed after challenging the chicken with Salmonella Typhimurium. Comparative differential expression results showed that following infection, a total of 3153 genes and 1787 genes were differentially expressed in the liver and spleen, respectively. The genes that were differentially expressed included interleukins, cytokines, NOS2, Avβ-defensins, toll-like receptors, and other immune-related gene families. Most of the genes and signaling pathways involved in the innate and adaptive immune responses against bacterial infection were significantly enriched in the Kashmir faverolla. Pathway analysis revealed that most of the enriched pathways were MAPK signaling pathway, NOD-like receptor signaling pathway, TLR signaling pathway, PPAR signaling pathway, endocytosis, etc. Surprisingly some immune-related genes like TLRs were upregulated in the susceptible chicken breed. On postmortem examination, the resistant birds showed small lesions in the liver compared to large necrotic lesions in susceptible birds. The patho...