Comparison of Transcriptomic Signatures between Monkeypox-Infected Monkey and Human Cell Lines

作者：Do Thi Minh Xuan, I‐Jeng Yeh, Chung-Che Wu, Che‐Yu Su, Hsin-Liang Liu, Chung-Chieh Chiao, Su‐Chi Ku, Jiazhen Jiang, Zhengda Sun, Hoang Dang Khoa Ta, Gangga Anuraga, Chih‐Yang Wang, Meng‐Chi Yen · 发表于：Journal of Immunology Research · 年份：2022 · DOI：10.1155/2022/3883822 · 被引用次数：47 · 研究领域：Poxvirus research and outbreaks、Herpesvirus Infections and Treatments、interferon and immune responses

Monkeypox virus (MPV) is a smallpox-like virus belonging to the genus Orthopoxvirus of the family Poxviridae. Unlike smallpox with no animal reservoir identified and patients suffering from milder symptoms with less mortality, several animals were confirmed to serve as natural hosts of MPV. The reemergence of a recently reported monkeypox epidemic outbreak in nonendemic countries has raised concerns about a global outburst. Since the underlying mechanism of animal-to-human transmission remains largely unknown, comprehensive analyses to discover principal differences in gene signatures during disease progression have become ever more critical. In this study, two MPV-infected in vitro models, including human immortal epithelial cancer (HeLa) cells and rhesus monkey (Macaca mulatta) kidney epithelial (MK2) cells, were chosen as the two subjects to identify alterations in gene expression profiles, together with co-regulated genes and pathways that are affected during monkeypox disease progression. Using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and MetaCore analyses, we discovered that elevated expression of genes associated with interleukins (ILs), G protein-coupled receptors (GPCRs), heat shock proteins (HSPs), Toll-like receptors (TLRs), and metabolic-related pathways play major roles in disease progression of both monkeypox-infected monkey MK2 and human HeLa cell lines. Interestingly, our analytical results also revealed that a cluster of differentia...