Integrated Acoustic Chip for Culturing 3D Cell Arrays

作者：Yong Luo, Hongxiao Gao, Mengyun Zhou, Long Xiao, Tailin Xu, Xueji Zhang · 发表于：ACS Sensors · 年份：2022 · DOI：10.1021/acssensors.2c01103 · 被引用次数：13 · 研究领域：Microfluidic and Bio-sensing Technologies、3D Printing in Biomedical Research、Microfluidic and Capillary Electrophoresis Applications

platform for clinical drug screening, but the bulky culture devices limit their application scenarios. Here, we demonstrate an integrated portable device that can realize contact-free construction of 3D cell spheroids. The interaction between the ultrasound generated by the portable device and the capillary results in periodic pressure nodes or anti-nodes, which lead to form a 3D cell array for cell culture. Such a 3D cell array pattern can be constructed in seconds and requires only 1 μL of cell samples. We further assessed the spheroids formed by the portable device and the impact of the acoustic field on spheroids and demonstrated the drug screening with assembled spheroids. More importantly, the integrated acoustic device can be further integrated with other components for more complex cell culture and all-round analysis. This portable and effective integrated device provides a new avenue for clinical biomedicine.