DNA-PAINT MINFLUX nanoscopy

作者：Lynn M. Ostersehlt, Daniel C. Jans, Anna Wittek, Jan Keller‐Findeisen, Kaushik Inamdar, Steffen J. Sahl, Stefan W. Hell, Stefan Jakobs · 发表于：Nature Methods · 年份：2022 · DOI：10.1038/s41592-022-01577-1 · 被引用次数：130 · 研究领域：Advanced biosensing and bioanalysis techniques、Advanced Fluorescence Microscopy Techniques、Advanced Electron Microscopy Techniques and Applications

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.