Changes in the m6A RNA methylome accompany the promotion of soybean root growth by rhizobia under cadmium stress

作者：Xue Han, Jialin Wang, Yu Zhang, Youlin Kong, Huiying Dong, Xuezhen Feng, Tianshu Li, Changjun Zhou, Jidong Yu, Dawei Xin, Qingshan Chen, Zhaoming Qi · 发表于：Journal of Hazardous Materials · 年份：2022 · DOI：10.1016/j.jhazmat.2022.129843 · 被引用次数：59 · 研究领域：Legume Nitrogen Fixing Symbiosis、RNA modifications and cancer

Cadmium (Cd) is the most widely distributed heavy metal pollutant in soil and has significant negative effects on crop yields and human health. Rhizobia can enhance soybean growth in the presence of heavy metals, and the legume–rhizobia symbiosis has been used to promote heavy-metal phytoremediation, but much remains to be learned about the molecular networks that underlie these effects. Here, we demonstrated that soybean root growth was strongly suppressed after seven days of Cd exposure but that the presence of rhizobia largely eliminated this effect, even prior to nodule development. Moreover, rhizobia did not appear to promote root growth by limiting plant Cd uptake: seedlings with and without rhizobia had similar root Cd concentrations. Previous studies have demonstrated a role for m6A RNA methylation in the response of rice and barley to Cd stress. We therefore performed transcriptome-wide m6A methylation profiling to investigate changes in the soybean RNA methylome in response to Cd with and without rhizobia. Here, we provide some of the first data on transcriptome-wide m6a RNA methylation patterns in soybean; m6A modifications were concentrated at the 3′ UTR of transcripts and showed a positive relationship with transcript abundance. Transcriptome-wide m6A RNA methylation peaks increased in the presence of Cd, and the integration of m6A methylome and transcriptome results enabled us to identify 154 genes whose transcripts were both differentially methylated and differ...