Cell Membrane and V 2 C MXene-Based Electrochemical Immunosensor with Enhanced Antifouling Capability for Detection of CD44

作者：Meiling Lian, Yuqing Shi, Liuxing Chen, Yongji Qin, Wei Zhang, Jingbo Zhao, Da Chen · 发表于：ACS Sensors · 年份：2022 · DOI：10.1021/acssensors.2c01215 · 被引用次数：72 · 研究领域：MXene and MAX Phase Materials、Advanced biosensing and bioanalysis techniques、Extracellular vesicles in disease

The inactive adsorption and interference of biomolecules in electrochemical biosensors is a topic of intense interest. Directly utilizing native cell membranes to endow electrochemical surfaces with antifouling and biocompatible features is a promising strategy, rather than attempting to synthetically replicate complex biological interface properties. In this study, we present a facial and sensitive sandwich-type antifouling immunoassay through platelet membrane/Au nanoparticle/delaminated V 2 C nanosheet (PM/AuNPs/d-V 2 C)-modified electrode as the substrate of sensing interface and methylene blue/aminated metal organic framework (MB@NH 2 -Fe-MOF-Zn) as an electrochemical signal probe. The biosensor perfectly integrates the high conductivity of AuNPs-loaded V 2 C MXene with the excellent loading property of NH 2 -Fe-MOF-Zn to improve the electrochemical sensing performance. In addition, the excellent antifouling properties of the homogeneous cell membrane can effectively prevent the non-specific adsorption of model proteins. The obtained antifouling biosensor possesses the capability of ultrasensitive detection of CD44 and CD44-positive cancer cell in complex liquids and exhibits good analytical performance for the analysis of CD44 with a linear range from 0.5 ng/mL to 500 ng/mL. This strategy of developing cell membrane-based biosensing systems with enhanced antifouling capability can be easily expanded to the construction of other complex biosensors, and the advanced biolo...