Bioinformatic analysis identifies potential key genes in the pathogenesis of age-related macular degeneration

作者：Zhiyue Wang, Xinyu Huang, Xiao Lv, Chao Chen, Shen Qu, Xiaoyu Ma, Li Zhang, Yanlong Bi · 发表于：Indian Journal of Ophthalmology · 年份：2022 · DOI：10.4103/ijo.ijo_3211_21 · 被引用次数：7 · 研究领域：Retinal Diseases and Treatments、Retinal Imaging and Analysis、Clusterin in disease pathology

Purpose: Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in older individuals. More studies focused on screening the genes, which may be correlated with the development of AMD. With advances in various technologies like multiple microarray datasets, researchers could identify differentially expressed genes (DEGs) more accurately. Exploring abnormal gene expression in disease status can help to understand pathophysiological changes in complex diseases. This study aims to identify the key genes and upstream regulators in AMD and reveal factors, especially genetic association, and the prognosis of the development of this disease. Methods: Data from expression profile GSE125564 and profile GSE29801 were obtained from the Gene Expression Omnibus (GEO) database. We analyzed DEGs using R software (version 3.6.3). Functional enrichment and PPI network analysis were performed using the R package and online database STRING (version 11.0). Results: We compared AMD with normal and found 68 up-regulated genes (URGs) and 25 down-regulated genes (DRGs). We also compared wet AMD with dry AMD and found 41 DRGs in dry AMD. Further work including PPI network analysis, GO classification, and KEGG analysis was done to find connections with AMD. The URGs were mainly enriched in the biological process such as DNA replication, nucleoplasm, extracellular exosome, and cadherin binding. Besides, DRGs were mainly enriched in these functions such as an integral compo...