Sympathetic nerve innervation and metabolism in ischemic myocardium in response to remote ischemic perconditioning

作者：Attila Kiss, Ping Wu, Michaela Schlederer, Patrick M. Pilz, Petra Lujza Szabó, Jingle Li, Lukas Weber, Chrysoula Vraka, Verena Pichler, Markus Mitterhauser, Xiaoli Zhang, Karin Zins, Dietmar Abraham, Sijin Li, Bruno K. Podesser, Marcus Hacker, Xiang Li · 发表于：Basic Research in Cardiology · 年份：2022 · DOI：10.1007/s00395-022-00946-3 · 被引用次数：13 · 研究领域：Cardiac Ischemia and Reperfusion、Cardiac Arrest and Resuscitation、Cardiac Imaging and Diagnostics

Abstract Sympathetic nerve denervation after myocardial infarction (MI) predicts risk of sudden cardiac death. Therefore, therapeutic approaches limit infarct size, improving adverse remodeling and restores sympathetic innervation have a great clinical potential. Remote ischemic perconditioning (RIPerc) could markedly attenuate MI-reperfusion (MIR) injury. In this study, we aimed to assess its effects on cardiac sympathetic innervation and metabolism. Transient myocardial ischemia is induced by ligature of the left anterior descending coronary artery (LAD) in male Sprague–Dawley rats, and in vivo cardiac 2-[ 18 F]FDG and [ 11 C] m HED PET scans were performed at 14–15 days after ischemia. RIPerc was induced by three cycles of 5-min-long unilateral hind limb ischemia and intermittent 5 min of reperfusion during LAD occlusion period. The PET quantitative parameters were quantified in parametric polar maps. This standardized format facilitates the regional radioactive quantification in deficit regions to remote areas. The ex vivo radionuclide distribution was additionally identified using autoradiography. Myocardial neuron density (tyrosine hydroxylase positive staining) and chondroitin sulfate proteoglycans (CSPG, inhibiting neuron regeneration) expression were assessed by immunohistochemistry. There was no significant difference in the mean hypometabolism 2-[ 18 F]FDG uptake ratio (44.6 ± 4.8% vs. 45.4 ± 4.4%) between MIR rats and MIR + RIPerc rats ( P > 0.05). However, the...