Gold Nanoparticle-Bridge Array to Improve DNA Hybridization Efficiency of SERS Sensors

作者：Xiaobin Huang, Weidong Zhao, Xiangyu Chen, Jinming Li, Haochen Ye, Cancan Li, Xiaomeng Yin, Xinyuan Zhou, Xuezhi Qiao, Zhenjie Xue, Tie Wang · 发表于：Journal of the American Chemical Society · 年份：2022 · DOI：10.1021/jacs.2c06623 · 被引用次数：58 · 研究领域：Gold and Silver Nanoparticles Synthesis and Applications、Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection

The interfacial mass transfer rate of a target has a significant impact on the sensing performance. The surface reaction forms a concentration gradient perpendicular to the surface, wherein a slow mass transfer process decreases the interfacial reaction rate. In this work, we self-assembled gold nanoparticles (AuNPs) in the gap of a SiO 2 opal array to form a AuNP-bridge array. The diffusion paths of vertical permeability and a microvortex effect provided by the AuNP-bridge array synergistically improved the target mass transfer efficiency. As a proof of concept, we used DNA hybridization efficiency as a research model, and the surface-enhanced Raman spectroscopy (SERS) signal acted as a readout index. The experimental verification and theoretical simulation show that the AuNP-bridge array exhibited rapid mass transfer and high sensitivity. The DNA hybridization efficiency of the AuNP-bridge array was 15-fold higher than that of the AuNP-planar array. We believe that AuNP-bridge arrays can be potentially applied for screening drug candidates, genetic variations, and disease biomarkers.