Integrative Multi-Omics Analysis of Identified Ferroptosis-Marker RPL8as a Candidate Oncogene Correlates with Poor Prognosis and ImmuneInfiltration in Liver Cancer

作者：Shunli Fan, Sai Zhang, Dejun Kong, Hao Wang, Yuan Shi, Zhenglu Wang, Hong Zheng · 发表于：Combinatorial Chemistry & High Throughput Screening · 年份：2022 · DOI：10.2174/1386207325666220823122942 · 被引用次数：10 · 研究领域：Ferroptosis and cancer prognosis、Immune cells in cancer、Clusterin in disease pathology

BACKGROUND: Liver Hepatocellular Carcinoma (LIHC) is characterized by high malignancy, poor prognosis, and high recurrence rate worldwide. The role of ferroptosis in tumorigenesis and progression has been confirmed in previous studies. However, the multi-omics analysis in liver cancer of ferroptosis-markers RPL8 remains to be elucidated. METHODS: In this analysis, the RPL8 mRNA expression was analyzed via the GEPIA, TIMER and UALCAN databases. In addition, we verified the mRNA expression of RPL8 by qRT-PCR experiment. The Kaplan-Meier plotter, UALCAN, TCGAportal and HPA databases were applied to evaluate RPL8 on prognosis and clinicopathological parameters. Moreover, we used TIMER and Kaplan-Meier plotter to analyze the correlation of RPL8 to immune cell infiltration and immune cell type markers to prognosis. In addition, networks and function enrichment between RPL8 coexpression genes were analyzed by GeneMANIA, cBioportal and Metascape databases. What's more, we used FerrDb and GEPIA databases to analyze the correlation of 23 Ferroptosis-related genes with RPL8. RESULTS: The mRNA expression of RPL8 was over-expressed in multiple cancers. In addition, transcription and translation levels of RPL8 in LIHC were significantly higher than normal tissues. Furthermore, higher expression of RPL8 was closely related to shorter OS in LIHC patients. The analysis of Kaplan-Meier plotter proved that RPL8 expression was related to stage, Sorafenib treatment, alcohol consumption and hepati...