[Retracted] Qingchang Wenzhong Decoction Alleviates DSS‐Induced Inflammatory Bowel Disease by Inhibiting M1 Macrophage Polarization In Vitro and In Vivo

作者：Qiongqiong Lu, Junxiang Li, Panghua Ding, Tangyou Mao, Lei Shi, Zhongmei Sun, Xiang Tan, Hui Jiang, Junying Dong, Yalan Li, Xiaojun Yang, Rui Shi · 发表于：BioMed Research International · 年份：2022 · DOI：10.1155/2022/9427076 · 被引用次数：10 · 研究领域：Immune cells in cancer、Inflammatory Bowel Disease、Gut microbiota and health

Background . An imbalance of macrophage M1/M2 polarization significantly influences the pathogenesis of inflammatory bowel disease. Qingchang Wenzhong decoction (QCWZD) has a proven therapeutic effect on patients with inflammatory bowel disease (IBD) and can significantly inhibit the inflammatory response in mice with colitis. However, its effect on macrophages during IBD treatment remains nebulous. Aim of the Study . Explore the mechanism underlying QCWZD effects in a dextran sulfate sodium (DSS)‐induced colitis mouse model in vivo and RAW264.7 cell in vitro by observing macrophage polarization dynamics. Methods . The main active components of QCWZD were determined using high‐performance liquid chromatography. Surface marker expression on M1‐type macrophages was analyzed using flow cytometry and immunofluorescence. The effect on inducible nitric oxide synthase (iNOS), interleukin‐6 (IL‐6), and tumor necrosis factor‐ α (TNF‐ α ) released by M1 type macrophages was determined using ELSA and RT‐PCR. The expression of key proteins in the JAK2/STAT3 signaling pathway was analyzed using western blotting. QCWZD cytotoxicity in macrophages was measured using CCK8 and Annexin V‐FITC/PI assays. Results . The main active components of QCWZD were berberine chloride, coptisine chloride, epiberberine chloride, gallic acid, ginsenoside Rg1, ginsenoside Rb1, indigo, indirubin, notoginsenoside R1, palmatine chloride, and 6‐curcumin. QCWZD markedly alleviated DSS‐induced colitis in mice, as r...