ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC

作者：Ting Zhu, Zhuoyu Xiao, Haoyu Yuan, Tian Hu, Taoyi Chen, Qi Chen, Mingkun Chen, Yang Jiankun, Qi-Zhao Zhou, Wenbin Guo, Kangyi Xue, Ming Xia, Jiming Bao, Cheng‐Ta Yang, Haifeng Duan, Hongyi Wang, Zhipeng Huang, Cundong Liu, Junhao Zhou · 发表于：Frontiers in Oncology · 年份：2022 · DOI：10.3389/fonc.2022.929838 · 被引用次数：27 · 研究领域：Ferroptosis and cancer prognosis、Immune cells in cancer、Clusterin in disease pathology

Background: ACO1 and IREB2 are two homologous cytosolic regulatory proteins, which sense iron levels and change iron metabolism-linked molecules. These two genes were noticeably decreased in kidney renal clear cell carcinoma (KIRC), which confer poor survival. Meanwhile, there is a paucity of information about the mechanisms and clinical significance of ACO1 and IREB2 downregulation in renal cancers. Methods: several bioinformatics platforms. Clinical and pathological information was utilized to stratify cohorts for comparison. Patient survival outcomes were evaluated using the Kaplan-Meier plotter, a meta-analysis tool. The correlations of ACO1 and IREB2 with ferroptosis were further evaluated in The Cancer Genome Atlas (TCGA)-KIRC database. Tumor immune infiltration was analyzed using the CIBERSORT, TIMER, and GEPIA data resources. ACO1 antagonist sodium oxalomalate (OMA) and IREB2 inhibitor sodium nitroprusside (SNP) was used to treat renal cancer ACHN cells together with sorafenib. Results: KIRC patients with low ACO1 or IREB2 contents exhibited a remarkably worse survival rate in contrast with those with high expression in Kaplan-Meier survival analyses. Meanwhile, ACO1 and IREB2 regulate autophagy-linked ferroptosis along with immune cell invasion in the tumor microenvironment in KIRC patients. Blocking the activation of these two genes by their inhibitors OMA and SNP ameliorated sorafenib-triggered cell death, supporting that ACO1 and IREB2 could be participated in its...