High-throughput microarray reveals the epitranscriptome-wide landscape of m6A-modified circRNA in oral squamous cell carcinoma

作者：Wei Zhao, Jingwen Liu, Jie Wu, Xiaozhou Ma, Xi Wang, Leyu Zhang, Zhe Han, Jianming Yang, Yameng Cui, Xin Hu, Jiayin Deng · 发表于：BMC Genomics · 年份：2022 · DOI：10.1186/s12864-022-08806-z · 被引用次数：24 · 研究领域：RNA modifications and cancer、Circular RNAs in diseases、Cancer-related molecular mechanisms research

Abstract Background Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N 6 -methyladenosine (m 6 A), greatly enrich the variety of RNAs metabolism. However, the m 6 A modification on circRNAs has yet to be addressed. Results Here, we report an epitranscriptome-wide mapping of m 6 A-modified circRNAs (m 6 A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m 6 A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m 6 A-circRNAs microarray, we found that m 6 A-circRNAs exhibited particular modification styles in OSCC, which was independent of m 6 A-mRNA. Besides, m 6 A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m 6 A-mRNA that in 3’-UTR or stop codon. Conclusion In conclusion, our work preliminarily demonstrates the traits of m 6 A-circRNAs, which may bring enlighten for the roles of m 6 A-circRNAs in OSCC.