Comprehensive analysis of m6A circRNAs identified in colorectal cancer by MeRIP sequencing

作者：Feng He, Qin Guo, Guo-xiu Jiang, Yan Zhou · 发表于：Frontiers in Oncology · 年份：2022 · DOI：10.3389/fonc.2022.927810 · 被引用次数：8 · 研究领域：RNA modifications and cancer、Circular RNAs in diseases、MicroRNA in disease regulation

Purpose To characterize the entire profile of m 6 A modifications and differential expression patterns for circRNAs in colorectal cancer (CRC). Methods First, High-throughput MeRIP-sequencing and RNA-sequencing was used to determine the difference in m 6 A methylome and expression of circRNA between CRC tissues and tumor-adjacent normal control (NC) tissues. Then, GO and KEGG analysis detected pathways involved in differentially methylated and differentially expressed circRNAs (DEGs). The correlations between m 6 A status and expression level were calculated using a Pearson correlation analysis. Next, the networks of circRNA-miRNA-mRNA were visualized using the Target Scan and miRanda software. Finally, We describe the relationship of distance between the m 6 A peak and internal ribosome entry site (IRES) and protein coding potential of circRNAs. Results A total of 4340 m 6 A peaks of circRNAs in CRC tissue and 3216 m 6 A peaks of circRNAs in NC tissues were detected. A total of 2561 m 6 A circRNAs in CRC tissues and 2129 m 6 A circRNAs in NC tissues were detected. Pathway analysis detected that differentially methylated and expressed circRNAs were closely related to cancer. The conjoint analysis of MeRIP-seq and RNA-seq data discovered 30 circRNAs with differentially m 6 A methylated and synchronously differential expression. RT-qPCR showned circRNAs (has_circ_0032821, has_circ_0019079, has_circ_0093688) were upregulated and circRNAs (hsa_circ_0026782, hsa_circ_0108457) were...