Enhancing control of multidrug-resistant plasmid and its host community with a prolonged thermophilic phase during composting

作者：Lei Shen, Tianlei Qiu, Yajie Guo, Min Gao, Haoze Gao, Guozhu Zhao, Xuming Wang · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.989085 · 被引用次数：7 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Composting and Vermicomposting Techniques、Antimicrobial agents and applications

The plasmid-mediated horizontal transfer of antibiotic resistance genes (ARGs) among bacteria facilitates the evolution and dissemination of antibiotic resistance. Broad-host-range plasmids can be transferred to different bacterial hosts in soil, plant rhizospheres, and wastewater treatment plants. Although composting is an effective way to convert organic waste into fertilizer and reduce some ARGs, few studies have focused on its effects on the spread of ARG-carrying plasmids and their bacterial host communities during composting. In this study, a fluorescently labeled Pseudomonas putida ( P. putida ) harboring a broad-host-range plasmid RP4 carrying three ARGs was inoculated into a raw material microcosm and composted with different durations of the thermophilic phase. The fate of the donor and RP4 in composting was investigated. The prolonged thermophilic composting removed 95.1% of dsRed and 98.0% of gfp , and it inhibited the rebound of P. putida and RP4 during the maturation phase. The spread potential of RP4 decreased from 10 −4 to 10 −6 transconjugants per recipient after composting. In addition, we sorted and analyzed the composition of RP4 recipient bacteria using fluorescence-activated cell sorting combined with 16S rRNA gene amplicon sequencing. The recipient bacteria of RP4 belonged to eight phyla, and Firmicutes, accounting for 75.3%–90.1%, was the dominant phylum in the transconjugants. The diversity and richness of the RP4 recipient community were significantl...