Species diversity analysis of commercial Mantidis Ootheca samples contaminated by store pests based on DNA metabarcoding

作者：Liuwei Xu, Xiaoying Zhang, Hua Guo, Yang Xia, Zhimei Xing, Wenzhi Yang, Jian Zhang, Xiaoxuan Tian · 发表于：Research Square · 年份：2022 · DOI：10.21203/rs.3.rs-1778531/v1 · 被引用次数：2 · 研究领域：Environmental DNA in Biodiversity Studies、Identification and Quantification in Food、Molecular Biology Techniques and Applications

Abstract Mantidis Ootheca (Sangpiaoxiao) is a typical multi-origin Chinese medicinal material. The Chinese Pharmacopoeia stipulates that the Mantidis Ootheca originates from Tenodera sinensis , Statilia maculate , and Hierodula patellifera . However, Mantidis Ootheca mainly relies on field collection, which leads to confusion of its actual origin in the market. As the clinical use of Mantidis Ootheca with unknown origin will pose potential risks to drug safety, it is necessary to survey the commercially available Mantidis Ootheca origin species. However, as the egg case of Mantis, the morphological characters of Mantidis Ootheca are limited and usually cannot serve as accurate identification tool. DNA barcoding, which is widely used in taxonomic studies of animals, is severely affected by the impact of storage pests and DNA degradation. Thus, this study aimed to analyze the origin diversity of 4,580 Mantidis Ootheca individuals contaminated by common store pests collected in the market using mini-barcode combined with DNA metabarcoding, and to provide a basis for quality control of Mantidis Ootheca. 37 Mantis ASVs and 9 Mantis MOTUs were identified through species delimitation, and the high level intraspecific diversity was depicted as haplotype network plot. Besides Tenodera sinensis and Hierodula patellifera as genuine sources defined in the Chinese Pharmacopoeia, Tenodera angustipennis was also the origin species of these Mantidis Ootheca samples.