Nasopharyngeal microbiota profiling of pregnant women with SARS-CoV-2 infection

作者：F. Crovetto, Marta Selma‐Royo, F. Crispi, Belén Carbonetto, Rosalia Pascal, Marta Larroya, Irene Casas, Marta Tortajada, Nuria Escudero, Carmen Muñoz‐Almagro, María Dolores Gómez‐Roig, Pedro González‐Torres, María Carmen Collado, E. Gratacós · 发表于：Scientific Reports · 年份：2022 · DOI：10.1038/s41598-022-17542-z · 被引用次数：20 · 研究领域：COVID-19 Impact on Reproduction、Gut microbiota and health、Reproductive tract infections research

We aimed to analyze the nasopharyngeal microbiota profiles in pregnant women with and without SARS-CoV-2 infection, considered a vulnerable population during COVID-19 pandemic. Pregnant women were enrolled from a multicenter prospective population-based cohort during the first SARS-CoV-2 wave in Spain (March-June 2020 in Barcelona, Spain) in which the status of SARS-CoV-2 infection was determined by nasopharyngeal RT-PCR and antibodies in peripheral blood. Women were randomly selected for this cross-sectional study on microbiota. DNA was extracted from nasopharyngeal swab samples, and the V3-V4 region of the 16S rRNA of bacteria was amplified using region-specific primers. The differential abundance of taxa was tested, and alpha/beta diversity was evaluated. Among 76 women, 38 were classified as positive and 38 as negative for SARS-CoV-2 infection. All positive women were diagnosed by SARS-CoV-2 IgG and IgM/IgA antibodies, and 14 (37%) also had a positive RT-PCR. The overall composition of the nasopharyngeal microbiota differ in pregnant women with SARS-CoV-2 infection (positive SARS-CoV-2 antibodies), compared to those without the infection (negative SARS-CoV-2 antibodies) (p = 0.001), with a higher relative abundance of the Tenericutes and Bacteroidetes phyla and a higher abundance of the Prevotellaceae family. Infected women presented a different pattern of microbiota profiling due to beta diversity and higher richness (observed ASV < 0.001) and evenness (Shannon index < 0...