All-fiber-transmission photometry for simultaneous optogenetic stimulation and multi-color neuronal activity recording

作者：Zhongyang Qi, Qingchun Guo, Shu Wang, Mingyue Jia, Xinwei Gao, Minmin Luo, Ling Fu · 发表于：Opto-Electronic Advances · 年份：2022 · DOI：10.29026/oea.2022.210081 · 被引用次数：13 · 研究领域：Photoreceptor and optogenetics research、Neural dynamics and brain function、Advanced Fluorescence Microscopy Techniques

Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo . However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine (DA) responses to unexpected rewards in the nucleus accumbens (NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca 2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-functi...