Cell-free fat extract improves ovarian function and fertility in mice with premature ovarian insufficiency

作者：Mengyu Liu, Dan Zhang, Xiaowei Zhou, Jingru Duan, Yanqin Hu, Wenjie Zhang, Qiang Liu, Bufang Xu, Aijun Zhang · 发表于：Stem Cell Research & Therapy · 年份：2022 · DOI：10.1186/s13287-022-03012-w · 被引用次数：72 · 研究领域：Reproductive Biology and Fertility、Ovarian function and disorders、Mesenchymal stem cell research

BACKGROUND: Premature ovarian insufficiency (POI) is a refractory disease that seriously affects the reproductive health of women and is increasing in incidence and prevalence globally. There is enormous demand to improve fertility in women with POI, while there is still lack of effective therapeutic methods in clinic. Cell-free fat extract (CEFFE) has been reported to contain thousands of active proteins which possess the ability to promote tissue repair in other diseases. In our study, we aimed to observe the efficacy and biosecurity of CEFFE on the repair of ovarian function and fertility of mice with POI and further explore the underlying mechanism. METHODS: and FSH in serum was measured by Enzyme-linked immunosorbent assay. Fertility was evaluated by the number of oocytes retrieved, the development of embryos in vitro and the litter size. Biosecurity of parent mice and their pups were examined by body mass and visceral index. The proliferation and apoptosis of cells in ovaries were examined by immunohistochemistry and transmission electron microscopy. Furthermore, the mRNA-Seq of mouse ovarian granulosa cells was performed to explore underlying mechanism of CEFFE. In vitro, KGN cell line and human primary ovarian granulosa cells (hGCs) were treated with 250 μM CTX for 48 h with/without CEFFE. The proliferative ability of cells was detected by cell counting kit-8 assay (CCK-8) and EDU test; the apoptosis of cells was detected by TUNEL and flow cytometry. RESULTS: and FSH ...