Reduced chemodiversity suppresses rhizosphere microbiome functioning in the mono-cropped agroecosystems

作者：Pengfa Li, Jia Liu, Muhammad Saleem, Guilong Li, Lu Luan, Meng Wu, Zhongpei Li · 发表于：Microbiome · 年份：2022 · DOI：10.1186/s40168-022-01287-y · 被引用次数：94 · 研究领域：Plant-Microbe Interactions and Immunity、Microbial Community Ecology and Physiology、Soil Carbon and Nitrogen Dynamics

Abstract Background Rhizodeposits regulate rhizosphere interactions, processes, nutrient and energy flow, and plant-microbe communication and thus play a vital role in maintaining soil and plant health. However, it remains unclear whether and how alteration in belowground carbon allocation and chemodiversity of rhizodeposits influences microbiome functioning in the rhizosphere ecosystems. To address this research gap, we investigated the relationship of rhizosphere carbon allocation and chemodiversity with microbiome biodiversity and functioning during peanut ( Arachis hypogaea ) continuous mono-cropping. After continuously labeling plants with 13 CO 2 , we studied the chemodiversity and composition of rhizodeposits, along with the composition and diversity of active rhizosphere microbiome using metabolomic, amplicon, and shotgun metagenomic sequencing approaches based on DNA stable-isotope probing (DNA-SIP). Results Our results indicated that enrichment and depletion of rhizodeposits and active microbial taxa varied across plant growth stages and cropping durations. Specifically, a gradual decrease in the rhizosphere carbon allocation, chemodiversity, biodiversity and abundance of plant-beneficial taxa (such as Gemmatimonas , Streptomyces , Ramlibacter , and Lysobacter ), and functional gene pathways (such as quorum sensing and biosynthesis of antibiotics) was observed with years of mono-cropping. We detected significant and strong correlations between rhizodeposits and rhiz...