Dietary Concentrate-to-Forage Ratio Affects Rumen Bacterial Community Composition and Metabolome of Yaks

作者：Simeng Yi, Dongwen Dai, Hao Wu, Shatuo Chai, Shujie Liu, Qingxiang Meng, Zhenming Zhou · 发表于：Frontiers in Nutrition · 年份：2022 · DOI：10.3389/fnut.2022.927206 · 被引用次数：65 · 研究领域：Ruminant Nutrition and Digestive Physiology、Gout, Hyperuricemia, Uric Acid、Reproductive Physiology in Livestock

Changes in dietary composition affect the rumen microbiota in ruminants. However, information on the effects of dietary concentrate-to-forage ratio changes on yak rumen bacteria and metabolites is limited. This study characterized the effect of three different dietary concentrate-to-forage ratios (50:50, C50 group; 65:35, C65 group; 80:20, C80 group) on yak rumen fluid microbiota and metabolites using 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC-MS) analyses. Rumen fermentation parameters and the abundance of rumen bacteria were affected by changes in the dietary concentrate-to-forage ratio, and there was a strong correlation between them. At the genus level, higher relative abundances of norank_f__F082 , NK4A214_group , Lachnospiraceae_NK3A20_group , Acetitomaculum , and norank_f__norank_o__Clostridia_UCG-014 were observed with a high dietary concentrate-to-forage ratio ( P < 0.05). Combined metabolomic and enrichment analyses showed that changes in the dietary concentrate-to-forage ratio significantly affected rumen metabolites related to amino acid metabolism, protein digestion and absorption, carbohydrate metabolism, lipid metabolism, and purine metabolism. Compared with the C50 group, 3-methylindole, pantothenic acid, D-pantothenic acid, and 20-hydroxy-leukotriene E4 were downregulated in the C65 group, while spermine and ribose 1-phosphate were upregulated. Compared to the C50 group, Xanthurenic acid, tyramine, ascorbic acid, D-glucuro...