Lipid metabolism and m6A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet

作者：Kefyalew Gebeyew, Chao Yang, Hui Mi, Yan Cheng, Tianxi Zhang, Fan Hu, Qiongxian Yan, Zhixiong He, Shaoxun Tang, Zhiliang Tan · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2022 · DOI：10.1186/s40104-022-00733-z · 被引用次数：21 · 研究领域：RNA modifications and cancer、Cancer-related gene regulation、Cancer-related molecular mechanisms research

Abstract Background Methionine or lysine has been reported to influence DNA methylation and fat metabolism, but their combined effects in N6-methyl-adenosine (m 6 A) RNA methylation remain unclarified. The combined effects of rumen-protected methionine and lysine (RML) in a low-protein (LP) diet on lipid metabolism, m 6 A RNA methylation, and fatty acid (FA) profiles in the liver and muscle of lambs were investigated. Sixty-three male lambs were divided into three treatment groups, three pens per group and seven lambs per pen. The lambs were fed a 14.5% crude protein (CP) diet (adequate protein [NP]), 12.5% CP diet (LP), and a LP diet plus RML (LP + RML) for 60 d. Results The results showed that the addition of RML in a LP diet tended to lower the concentrations of plasma leptin ( P = 0.07), triglyceride ( P = 0.05), and non-esterified FA ( P = 0.08). Feeding a LP diet increased the enzyme activity or mRNA expression of lipogenic enzymes and decreased lipolytic enzymes compared with the NP diet. This effect was reversed by supplementation of RML with a LP diet. The inclusion of RML in a LP diet affected the polyunsaturated fatty acids (PUFA), n-3 PUFA, and n-6 PUFA in the liver but not in the muscle, which might be linked with altered expression of FA desaturase-1 ( FADS1 ) and acetyl-CoA carboxylase ( ACC ). A LP diet supplemented with RML increased ( P < 0.05) total m 6 A levels in the liver and muscle and were accompanied by decreased expression of fat mass and obesity-...