Advances in glycopeptide enrichment methods for the analysis of protein glycosylation over the past decade

作者：Jiajia Li, Jie Zhang, Mingming Xu, Zeren Yang, Shuang Yue, Wanlong Zhou, Chunshan Gui, Haiyang Zhang, Shuwei Li, Perry G. Wang, Shuang Yang · 发表于：Journal of Separation Science · 年份：2022 · DOI：10.1002/jssc.202200292 · 被引用次数：40 · 研究领域：Glycosylation and Glycoproteins Research、Advanced Proteomics Techniques and Applications、Genomics and Phylogenetic Studies

Advances in bioanalytical technology have accelerated the analysis of complex protein glycosylation, which is beneficial to understand glycosylation in drug discovery and disease diagnosis. Due to its biological uniqueness in the course of disease occurrence and development, disease-specific glycosylation requires quantitative characterization of protein glycosylation. We provide a comprehensive review of recent advances in glycosylation analysis, including workflows for glycoprotein digestion, glycopeptide separation and enrichment, and mass spectrometry sequencing. We specifically focus on different strategies for glycopeptide enrichment through physical interaction, chemical oxidation, or metabolic labeling of intact glycopeptides. Recent advances and challenges of O-glycosylation analysis are presented, and the development of improved enrichment methods combining different proteases to analyze O-glycosylation is also proposed.